Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Correlation Between Serum S100A12 Levels and Other Variables Using Spearman Correlation Coefficient in Acute Intracerebral Hemorrhage

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Analysis regarding predictive value of serum S100A12 levels for early neurologic deterioration after acute intracerebral hemorrhage. Discriminatory ability was assessed under receiver operating characteristic curve.

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Factors Associated with Early Neurologic Deterioration in Acute Intracerebral Hemorrhage

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Relationship between serum S100A12 levels and modified Rankin scale score among patients with acute intracerebral hemorrhage. Comparison was done using Kruskal–Wallis H -test. In boxplot, ***P < 0.001 compared with modified Rankin scale score 0, ### P < 0.001 compared with modified Rankin scale score 1, &&& P < 0.001 compared with modified Rankin scale score 2, and $$$ P < 0.001 compared with modified Rankin scale score 3.

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques: Modification, Comparison

Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Relationship between serum S100A12 level and modified Rankin scale score following acute intracerebral hemorrhage. Bivariate correlation was assessed using Spearman correlation coefficient and association was summarized as r value.

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques: Modification

Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Analysis with respect to predictive ability of serum S100A12 levels for 3-month poor outcome after acute intracerebral hemorrhage. Discriminatory capability was evaluated using receiver operating characteristic curve analysis. A poor outcome was referred to as modified Rankin scale score above 2.

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques: Modification

Journal: Neuropsychiatric Disease and Treatment

Article Title: High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage

doi: 10.2147/NDT.S337041

Figure Lengend Snippet: Factors Associated with 3-Month Poor Outcome in Acute Intracerebral Hemorrhage

Article Snippet: Serum S100A12 levels were quantified in duplicate using a commercially available enzyme-linked immunosorbent assay (Cusabio Biotech Co., Wuhan, Hubei Province, P.R.

Techniques: Functional Assay

Journal: Nutrients

Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

doi: 10.3390/nu12123659

Figure Lengend Snippet: The median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL) between non-diabetic, pre-diabetic and diabetic subjects.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

Techniques: Concentration Assay

Journal: Nutrients

Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

doi: 10.3390/nu12123659

Figure Lengend Snippet: Comparison of the median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL) in non-diabetes, pre-diabetes and diabetes, grouped in different ethnic groups.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

Techniques: Comparison, Concentration Assay

Journal: Nutrients

Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

doi: 10.3390/nu12123659

Figure Lengend Snippet: Ethnic comparison of median serum concentration of α-tocopherol (μmol/L), corrected serum concentration of α-tocopherol, HOMA2 IR, HOMA2%B and serum RAGE concentration (pg/mL), grouped in non-diabetes, pre-diabetes and diabetes.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

Techniques: Comparison, Concentration Assay

Journal: Nutrients

Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

doi: 10.3390/nu12123659

Figure Lengend Snippet: The association factors of the different stages of development of type 2 diabetes with corrected serum α-tocopherol concentration, HOMA2 IR, HOMA2%B and serum RAGE concentration.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

Techniques: Concentration Assay

Journal: Nutrients

Article Title: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

doi: 10.3390/nu12123659

Figure Lengend Snippet: Correlation of corrected serum α-tocopherol concentration with HOMA2 IR, HOMA2%B and serum RAGE Concentration.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure serum insulin levels (Elabscience E-EL-H2665, Houston, TX, USA) and serum RAGE levels (Elabscience E-EL-H0295, Houston, TX, USA) based on the manufacturer’s protocol.

Techniques: Concentration Assay

Journal: Gene therapy

Article Title: Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.

doi: 10.1038/sj.gt.3300648

Figure Lengend Snippet: Figure 1 Expression of Epo following infection of HT1080 cells. Super- natant was harvested from cells 48 h after infection and analyzed by ELISA. Titers of rAAV-cm-Epo are indicated on X-axis. Bar marked lep- tin represents background levels of Epo secreted from cells infected with 5 × 109 particles of an irrelevant virus (rAAV-m-leptin).

Article Snippet: The levels of Epo were determined from monkey plasma or mouse serum using the R&D Systems ELISA Quantikine kit (R&D Systems).

Techniques: Expressing, Infection, Enzyme-linked Immunosorbent Assay, Virus

Journal: Gene therapy

Article Title: Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.

doi: 10.1038/sj.gt.3300648

Figure Lengend Snippet: Figure 3 Titer of anti-Epo antibodies in C57BL/6 mice following rAAV- cm-Epo administration. Antibodies were measured as described in the experimental protocol. For saline-treated mice, sera were pooled before ELISA, sera from rAAV-treated mice were measured individually. Posi- tive control (+ control) represents sera from Cynomolgus monkey-Epo plasmid DNA injected BALB/c mice which had previously been shown to have anti-cm-Epo antibodies. Titer is defined as the dilution of serum required to reduce the signal to levels obtained in wells containing dilution buffer alone.

Article Snippet: The levels of Epo were determined from monkey plasma or mouse serum using the R&D Systems ELISA Quantikine kit (R&D Systems).

Techniques: Saline, Enzyme-linked Immunosorbent Assay, Control, Plasmid Preparation, Injection

Journal: Gene therapy

Article Title: Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.

doi: 10.1038/sj.gt.3300648

Figure Lengend Snippet: Figure 2 Effect of rAAV-cm-Epo administration in C57BL/6 mice. (a) Serum Epo concentration (±s.e.m.), as measured by ELISA, following rAAV administration. At all time-points, the saline-injected mice had undetectable levels of serum Epo and are not included on this graph. (b) The average hematocrit of four mice injected with either rAAV-cm-Epo or saline. Although error bars are included, they are obscured by the plot symbols The 0 week time-points represent the average baseline hematocrit and serum Epo concentrations for untreated C57BL/6 mice.

Article Snippet: The levels of Epo were determined from monkey plasma or mouse serum using the R&D Systems ELISA Quantikine kit (R&D Systems).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Saline, Injection

Journal: Gene therapy

Article Title: Adeno-associated virus-mediated delivery of erythropoietin leads to sustained elevation of hematocrit in nonhuman primates.

doi: 10.1038/sj.gt.3300648

Figure Lengend Snippet: Figure 4 Response of baboons to administration of rAAV-cm-Epo. (a) The plasma Epo levels as measured by ELISA. (b) The hematocrits of two baboons at times before injection (negative numbers) and after injection. To prevent stroke, 34 or 40 ml of blood was removed from baboon 2 at weeks 11 and 13.

Article Snippet: The levels of Epo were determined from monkey plasma or mouse serum using the R&D Systems ELISA Quantikine kit (R&D Systems).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Injection